• One-stop Service

    One-stop service includes cell pool screening, high yield cell selection, cell amplification, stability prediction, and delivery of cell banks and relevant files. 

  • Customer Cloud Service

    The customer cloud service remotely provides customers with real-time image scanning and image analysis, can locate the target cells in a short time.


  • Pay after Service

    Qualified customers can pay after service without any risks.

Developed by Great Bay Bio, Klone4.0 is a biotechnological service platform focusing on cell line development. It can screen hundreds of thousands of cells at one time, obtain cell images, and quickly analyze and locate high-yield cells through AI technology and unique cell selecting technology. It can obtain high-yield and high-quality single cells in 4 hours. 

Platform Introduction















4 小时单克隆化

Much higher Qp than the traditional way


Obtain high yield single cells in a short time

High Yield

Save up to 80 % workload


Develop cell lines in 1.5~2 months


Monoclone cells selection in 4 hours

Platform value


More cell line candidates

Stability Prediction

Original technology


Predict the cell line stability in 15 days

Clonality in 4 hours

Making cell line development easier and more efficient

Process Flow


Platform Introduction

Klone4.0 is a cell line development platform of Great Bay Bio, which enables intelligent application of cell line construction through AI and precise cell selection technology.

One-stop Service

Customers experience the full value of the platform

Business Model


·  High yield cell lines

·  More cell line candidates

·  Cell line stability prediction

Platform Values




Great Bay Bio attaches great importance to the frequently asked questions by clients

Q. How long does it take for Klone4.0 to develop the monoclonal cell line?


A:If customers provide cell pool, it takes1.5 months;

      If customers provide sequence, 2.5 months is needed.




Q. Is the monoclonal yield of Klone4.0 higher than that of traditional methods?


A:Compared with traditional methods, Klone4.0 can screen far more cells. Therefore, usually K4 can abtain higher yield cells if it stars with the same cell pool.




Q. Will the cells displace during the high-throughput photographing?

A:It has been proved that the cell positions can remain traceable, which can meet the experiment requirements.




Q. Will selecting technologies damage the cells?


A:Experiments have showed that the technologies won’t damage any cells and the monoclonal formation rate is equivalent to that of traditional technologies.




Q. How to determine monoclonality?


A:Klone4.0 platform uses high-throughput photographing technologies to verify the monoclonality.




Q. Does Klone 4.0 have independent intellectual property rights?


A:The key technologies involved are all independently developed by GBB. We have a sound intellectual property matrix and IP layouts at domestic and overseas.




Q. How to authorize the vector cell line?


A:Cell lines provided by customers needn’t be authorized any more. If customers prefer to use the contracted cell lines of Klone4.0, the license fee shall be calculated separately.

Frequently Asked Questions