Cell line development (CLD) plays a pivotal role in determining the robustness and cost-effectiveness of downstream bioprocessing development. Great Bay Bio developed a proprietary AlfaCell platform that applies Site-Specific Integration (SSI) technology and AI-enabled cell selection to accurately insert the target genes into specific “hot spot” within the genome of a host cell line. AlfaCell platform significantly reduces time, effort, and cost that are associated with the development of high-performance mammalian cell lines.