Transient Expression Kit
Powered by the Fluorescent Site-specific Integration Cell line Development Platform (AlfaCell), Alfaexpress transient expression kit offers a one-stop protein expression solution for CHO-K1 cells. It achieves yields significantly higher than commercial transient expression kit, using the same cell line for both transient and stable development. This ensures seamless quality consistency from early research to CMC, bridging the gap to large-scale manufacturing.
Transient Expression in CHO-K1 Cell Line
The Alfaexpress transient expression kit leverages site-specific integration technology for rapid transient protein expression in CHO-K1 cells. Monoclonal antibody yields reach 300-1,000 mg/L by day 14—far exceeding commercial kit performance—while maintaining high cell viability. Ideal for fast protein sample generation and early evaluation of expression, post-translational modification, and stability.
One Cell Line for Both Transient and Stable Expression
The Alfaexpress transient expression kit enables seamless use of the same CHO-K1 cell line for both transient and stable expression, ensuring consistent product quality. This bridges early research and CMC, reducing efficacy gaps and lowering development risk.
Product Information
| Name | CHO Cell Culture Medium | Expression Medium | Feed | AC Enhancer | Trans A | Trans B | Trans G | GBB-lp02 | GBB-p10 |
| Product | Passage & Expansion Medium | Transfection & Expression Medium | Feed Medium | Expression Enhancer | Transfection Reagent | Positive Control Plasmid | Empty Vector Plasmid | ||
| Product Code | NF3370 | EM3302 | EM3302 Feed | AC Enhancer 01 | Trans A01 | Trans B01 | Trans G01 | GBB-Ip02 | GBB-p10 |
| Quantity | 1000 ml | 1000 ml | 200 ml | 2 ml*2 | 1 ml*2 | 1.75 ml*4 | 0.3 ml | 0.12 ml | 25 μl |
Case Study
Superior Titers
Enhanced Cell Viability
Cell Viability
Alfaexpress delivered a faster and higher titer increase for mAb-6, reaching nearly 1,000 mg/L by day 14.
Seamless CMC Transition
